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Carboxylesterase, also known as carboxyl ester hydrolase is a member of the hydrolase superfamily of enzymes. Pig liver esterase catalyzes enantioselective conversion of an ester to a carboxylic acid. The molecular weight is found to be 168 kDa. It is a serine enzyme with two active sites on each molecule, which dissociates into active half-molecules in the presence of dilute acid or concentrated salts. Esterase from porcine liver has been used to unmask the fluorescence in ultraviolet-visible spectroscopy. Porcine liver esterase is used to catalyze the hydrolysis of pentaacetyl catechin and epicatechin for use in pharmaceutical and industrial applications. Pig liver esterase is commonly used for kinetic resolutions and assymetric synthesis in organic chemistry.
One unit will hydrolyze 1.0 μmole of ethyl butyrate to butyric acid and ethanol per min at pH 8.0 at 25 °C.
| CAS Number | 9016-18-6 |
| Form | Lyophilized powder |
| Solubility (25°C) | Water |
| Storage | -20°C |
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