All AbMole products are for research use only, cannot be used for human consumption.
NPPB is a blocker of the outwardly rectifying chloride channel (ORCC) with IC50 of 80 nM. NPPB has been shown to activate the GPR35-Gαi/o and GPR35-Gα16 pathways in HEK293 cells, inducing intracellular calcium mobilization.
Acta Med Okayama. 2022 Aug;76(4):415-421.
5-Nitro-2-(3-phenylpropylamino) Benzoic Acid Inhibits the Proliferation and Migration of Lens Epithelial Cells by Blocking CaMKII Signaling
NPPB purchased from AbMole
Int J Mol Med. 2021 Apr;47(4):59.
5‑Nitro‑2‑(3‑phenylpropylamino) benzoic acid induces apoptosis of human lens epithelial cells via reactive oxygen species and endoplasmic reticulum stress through the mitochondrial apoptosis pathway
NPPB purchased from AbMole
 |
Source | Int J Mol Med (2021). Figure 2. NPPB (Abmole Bioscience Inc., USA) |
| Method | Mitochondrial membrane potential analysis | |
| Cell Lines | HLECs | |
| Concentrations | 50 µM | |
| Incubation Time | 24 h | |
| Results | JC‑1 aggregated in the mitochondria in the control group, with a ratio of 0.15±0.027, while the NPPB‑treated group exhibited a significantly higher ratio (0.59±0.080) since the monomeric form of JC‑1 was present in the cytosol |
 |
Source | Int J Mol Med (2021). Figure 1. NPPB (Abmole Bioscience Inc., USA) |
| Method | Cell viability assay | |
| Cell Lines | HLECs | |
| Concentrations | 10, 50, 100 and 200 µM | |
| Incubation Time | 24 h | |
| Results | Fig. 1 shows that NPPB significantly inhibited the viability of HLECs in a dose‑dependent manner, with an IC50 value of 53.36±0.1% (P<0.01 vs. 0 µM). These results suggested that NPPB decreased cell survival rate and inhibited the viability of HLECs. A concentration of 50 µM NPPB was used in subsequent experiments. |
| Molecular Weight | 300.31 |
| Formula | C16H16N2O4 |
| CAS Number | 107254-86-4 |
| Solubility (25°C) | DMSO ≥ 60 mg/mL |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
| Related Chloride Channel Products |
|---|
| Atractyloside potassium salt
Atractyloside potassium salt is a highly effective and specific inhibitor of mitochondrial ADP/ATP transport. Atractyloside potassium salt inhibited the chloride channel in rat heart mitochondrial membrane. Atractyloside potassium salt is a toxic diterpenoid glycoside, which can be isolated from the fruit of Atractyloside. |
| CaCCinh-A01
CaCCinh-A01 is a calcium-activated chloride channel (CaCC) inhibitor. |
| Eact
Eact is a activator of Ca 2+-activated Cl - channel transmembrane protein 16A (TMEM16A). |
| T16Ainh-A01
T16Ainh-A01 is a Ca2+-activated Cl- channel transmembrane protein 16A (TMEM16A) inhibitor. |
| Ani9
Ani9 is a potent and highly selective anoctamin1 (ANO1)/transmembrane protein 16A (TMEM16A) inhibitor that completely inhibited ANO1 chloride current with submicromolar potency. |
All AbMole products are for research use only, cannot be used for human consumption or veterinary use. We do not provide products or services to individuals. Please comply with the intended use and do not use AbMole products for any other purpose.
Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2024 AbMole BioScience. All Rights Reserved.
