All AbMole products are for research use only, cannot be used for human consumption.
NAD+, nicotinamide adenine dinucleotide, is a coenzyme that transfers hydrogen ions and is formed by the coupling of ribosylnicotinamide 5'-diphosphate to adenosine 5'-phosphate via pyrophosphate bonding. NAD+ is the oxidized form of NADH. NAD+ is a coenzyme that is formed by the coupling of ribosylnicotinamide 5'-diphosphate to adenosine 5 '-phosphate coupled by pyrophosphate bonding.
Nat Commun. 2021 Mar 8;12(1):1513.
Hyperproduction of 3-hydroxypropionate by Halomonas bluephagenesis
NAD+ purchased from AbMole
 |
Source | Nat Commun (2021 Mar). Figure 1. NAD+ (Abmole Bioscience) |
| Method | Protein expression and enzyme activities | |
| Cell Lines | Plasmids p90, p91, p92, and p98 | |
| Concentrations | 2.0 mM | |
| Incubation Time | 1 min | |
| Results | 3HP production via metabolic engineering started from 1,3-propanediol could avoid the paradox between glycerol dehydratase and aldehyde dehydrogenase activities, which generate from the imbalance of vita min B12 synthesis and NAD+ regeneration. |
| Cell Experiment | |
|---|---|
| Cell lines | MKN-45 and MCF-7 cells |
| Preparation method | For MKN-45 cells, and MCF-7 cells, treatment of the cells with as low as 1 µM NAD+ significantly decreased the survival of the cells. Our Trypan blue assays also showed that NAD+ treatment for 24 or 48 hrs significantly decreased the number of surviving C6 glioma cells |
| Concentrations | 1 µM |
| Incubation time | 24 or 48 h |
| Animal Experiment | |
|---|---|
| Animal models | Male C57BL/6N mice |
| Formulation | saline |
| Dosages | 1 mg/kg |
| Administration | Intraperitoneal injection |
| Molecular Weight | 663.43 |
| Formula | C21H27N7O14P2 |
| CAS Number | 53-84-9 |
| Solubility (25°C) | Water 50 mg/mL |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
[2] Luis Rajman, et al. Therapeutic Potential of NAD-Boosting Molecules: The In Vivo Evidence
[5] G Sultani, et al. NAD + : A key metabolic regulator with great therapeutic potential
| Related Enzymes & Coenzymes Products |
|---|
| Nattokinase
Nattokinase is an enzyme extracted and purified from a Japanese food called nattō. |
| Papain
Papain is a cysteine protease of the peptidase C1 family. |
| Proteinase K
Proteinase K is used for digesting various proteins and common molecular biology, cell biology and other related experiments, such as genomic DNA extraction, enzyme digestion and removal. |
| NADH disodium salt
NADH disodium salt is a coenzyme of a large number of oxidoreductases. NADH disodium salt is a coenzyme that functions as a regenerating electron donor in catabolic processes including glycolysis, beta-oxidation and the citric acid cycle. |
| Lipoamide
Lipoamide is a coenzyme, which transfer acetyl and hydrogen in Pyruvate deacylation oxidation Process, used for pharmaceuticals. |
All AbMole products are for research use only, cannot be used for human consumption or veterinary use. We do not provide products or services to individuals. Please comply with the intended use and do not use AbMole products for any other purpose.
Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2024 AbMole BioScience. All Rights Reserved.
