All AbMole products are for research use only, cannot be used for human consumption.
Fatostatin A is a cell permeable inhibitor of SREBP activation, it inhibits cancer cell proliferation by affecting mitotic microtubule spindle assembly and cell division.
| Cell Experiment | |
|---|---|
| Cell lines | CHO-K1 cells |
| Preparation method | CHO-K1 cells are plated out onto a 96-well plate in medium A. The cells are transiently cotransfected with pCMV-PLAP-BP2, pCMV-SCAP, and pAc-b-gal, using Lipofectamine reagent. After incubation for 5 hr, the cells are washed with PBS and then incubated in medium B, in the absence or presence of fatostatin (20 μM) or sterols (10 μg/mL cholesterol and 1 μg/mL 25-hydroxycholesterol). After 20 hr of incubation, an aliquot of the medium is assayed for secreted alkaline phosphatase activity. The cells in each well are lysed and used for measurement of b-galactosidase activities. |
| Concentrations | 20 μM |
| Incubation time | 20 h |
| Animal Experiment | |
|---|---|
| Animal models | Four-to-five-week-old homozygous male obese (ob/ob) mice |
| Formulation | 10% DMSO in PBS |
| Dosages | 30 mg/kg; 150 uL |
| Administration | i.p. injection |
| Molecular Weight | 294.41 |
| Formula | C18H18N2S |
| CAS Number | 125256-00-0 |
| Solubility (25°C) | DMSO ≥ 30 mg/mL |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
| Related FAS Products |
|---|
| C75
C75 is a novel, potent synthetic inhibitor of fatty acid synthase (FAS). C75 inhibits prostate cancer cells PC3 with an IC50 of 35 μM. C75 is a potent CPT1A activator. |
| Orlistat
Orlistat is an irreversible inhibitor of pancreatic and gastric lipases with IC50 of 122 ng/ml for PL from human duodenal juice. |
| Betulin
Betulin is a specific, small molecule inhibitor of SREBP (Sterol Regulatory Element-Binding Proteins). |
| Pseudoprotodioscin
Pseudoprotodioscin, a furan glucoside, inhibits sREBP1/2 and microRNA 33A/B levels and reduces the expression of genes associated with cholesterol and triglyceride synthesis. |
| Albaspidin-AP
Albaspidin AP inhibited fatty acid synthase (FAS) with IC50 value of 71.7 μM. Fatty acid synthase is emerging as a potential therapeutic target for cancer and obesity. |
All AbMole products are for research use only, cannot be used for human consumption or veterinary use. We do not provide products or services to individuals. Please comply with the intended use and do not use AbMole products for any other purpose.
Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2024 AbMole BioScience. All Rights Reserved.
