D-Luciferin potassium salt

Biological Activity

D-Luciferin potassium salt is the natural specific substrate for firefly luciferase (FLuc) and one of the most commonly used dyes in bioluminescence and in vivo imaging experiments. D-Luciferin potassium salt is catalysed by FLuc into luciferin in the presence of ATP, magnesium ions, and oxygen, emitting fluorescence with a peak wavelength of approximately 560 nm. In experiments, by integrating the FLuc gene as a reporter element into target cells (such as tumour cells, stem cells, or pathogenic microorganisms) or transgenic animals, followed by intraperitoneal or intravenous injection of D-luciferin potassium salt, real-time, non-invasive tracking and quantitative analysis of these targets can be achieved using imaging equipment.

In Vitro: D-luciferin is the natural substrate of the enzyme luciferase (Luc), that catalyzes the production of the typical yellowgreen light of fireflies.

In Vivo: The total signal intensity is plotted against the time after D-luciferin injection to generate a time-intensity curve. In addition to the peak signal, the signals at fixed time points (5, 10, 15, and 20 min) after D-luciferin injection are determined as alternatives to the peak signal.

Product Citations

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  Cell Metab. 2025 Apr 07.

  Targeted tumor therapy with L-cyst(e)ine-addicted bacteria-nanodrug biohybrids
  D-Luciferin potassium salt purchased from AbMole

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  Dev Cell. 2025 Feb 08.

  TaANK-TPR1 enhances wheat resistance against stripe rust via controlling gene expression and protein activity of NLR protein TaRPP13L1
  D-Luciferin potassium salt purchased from AbMole

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  Stress Biol. 2025 Jun 16;5(1):42.

  Cellulose synthase TaCESA7 negatively regulates wheat resistance to stripe rust by reducing cell wall lignification
  D-Luciferin potassium salt purchased from AbMole

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  Bioact Mater. 2024 Dec 10;46:82-96.

  Lipidomic analysis of plant-derived extracellular vesicles for guidance of potential anti-cancer therapy
  D-Luciferin potassium salt purchased from AbMole

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  J Exp Clin Cancer Res. 2024 Aug 13;43(1):224.

  Galectin-3 induces pathogenic immunosuppressive macrophages through interaction with TREM2 in lung cancer
  D-Luciferin potassium salt purchased from AbMole

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  Plant J. 2024 Nov 01.

  Fast and simple fluorometric measurement of phloem loading exposes auxin-dependent regulation of Arabidopsis sucrose transporter AtSUC2
  D-Luciferin potassium salt purchased from AbMole

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  Int J Pharm. 2024 Aug 05.

  The peptide-based bispecific CAR T cells target EGFR and tumor stroma for effective cancer therapy
  D-Luciferin potassium salt purchased from AbMole

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  bioRxiv. 2024 Oct 10.

  Circadian clock features define novel breast cancer subtypes and shape drug sensitivity
  D-Luciferin potassium salt purchased from AbMole

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  Patent. CN118256447A 2024 Jun 28.

  Patent. CN118256447A
  D-Luciferin potassium salt purchased from AbMole

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  Cell Res. 2023 Jun;33(6):464-478.

  SCUBE2 mediates bone metastasis of luminal breast cancer by modulating immune-suppressive osteoblastic niches
  D-Luciferin potassium salt purchased from AbMole

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  J Transl Med. 2023 Jun 12;21(1):383.

  Inhalable CAR-T cell-derived exosomes as paclitaxel carriers for treating lung cancer
  D-Luciferin potassium salt purchased from AbMole

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  Plant Physiol. 2022 Mar 14;kiac112.

  TaBln1, a member of the Blufensin family, negatively regulates wheat resistance to stripe rust by reducing Ca2+ influx
  D-Luciferin potassium salt purchased from AbMole

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  Front Bioeng Biotechnol. 2022 Jun 17;10:928169.

  Reforming the Chimeric Antigen Receptor by Peptide Towards Optimized CAR T Cells With Enhanced Anti-Cancer Potency and Safety
  D-Luciferin potassium salt purchased from AbMole

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  Adv Sci (Weinh). 2021 Jan.

  Chemotherapy‐Enriched THBS2‐Deficient Cancer Stem Cells Drive Hepatocarcinogenesis through Matrix Softness Induced Histone H3 Modifications
  D-Luciferin potassium salt purchased from AbMole

Protocol (for reference only)

Cell Experiment
Cell lines	HEK293 cells
Preparation method	HEK293/eLuc cells grown on white 96-well microtiter plates coated with poly-l-lysine were transiently transfected with several transporters using Lipofectamine 2000. Cells in each well were preincubated in 0.1 ml of uptake buffer for 5 min. Bioluminescence assays were initiated by replacing the preincubation buffer with 0.1 ml of buffer containing d-luc. The luminescence intensity (LI) of BL was measured using a microplate luminometer.
Concentrations	0-2 µM
Incubation time	15 min

Animal Experiment
Animal models	Nude mice
Formulation	Saline
Dosages	150 mg/kg
Administration	i.p.

Chemical Information

Molecular Weight	318.41
Formula	C11H7KN2O3S2
CAS Number	115144-35-9
Solubility (25°C)	Water 25 mg/mL
Storage	-20°C, protect from light, sealed

References

[1] Inoue Y, et al. Int J Biomed Imaging. Timing of imaging after d-luciferin injection affects the longitudinal assessment of tumor growth using in vivo bioluminescence imaging.