Certificate of Analysis

 

Product Name: D-Luciferin potassium salt
Catalog Number: M8873
CAS Number: 115144-35-9

Physical and Chemical Properties
Formula: C11H7KN2O3S2
Molecular Weight: 318.41
Solubility: Water 25 mg/mL
Storage: -20°C, protect from light, sealed
Chemical Structure: D-Luciferin potassium salt Structure
Analytical Data
QC Standard: 99.28%
H-NMR: Consistent with structure
Product Information
Description: D-Luciferin potassium salt is the natural specific substrate for firefly luciferase (FLuc) and one of the most commonly used dyes in bioluminescence and in vivo imaging experiments. D-Luciferin potassium salt is catalysed by FLuc into luciferin in the presence of ATP, magnesium ions, and oxygen, emitting fluorescence with a peak wavelength of approximately 560 nm. In experiments, by integrating the FLuc gene as a reporter element into target cells (such as tumour cells, stem cells, or pathogenic microorganisms) or transgenic animals, followed by intraperitoneal or intravenous injection of D-luciferin potassium salt, real-time, non-invasive tracking and quantitative analysis of these targets can be achieved using imaging equipment.
   
Stability and Solubility Advice: Information concerning product stability, particularly in solution, has rarely been reported and in most cases we can only offer a general guide.
We recommend that stock solutions, once prepared, are stored aliquoted in tightly sealed vials and used within 1 month. Avoid repeated freeze and thaw cycles. Storage conditions for some special products should refer to their storage details.