Mitomycin C

Biological Activity

Mitomycin C (Ametycine) is an antineoplastic antibiotic by inhibiting DNA synthesis. Mitomycin C (Ametycine) is a DNA cross-linking agent and induces DNA damaging. Mitomycin C is an ADC Cytotoxin and induces apoptosis.

Product Citations

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  Comput Methods Programs Biomed. 2025 Jul 28; .

  Discovery of novel microtubule destabilizing agents via virtual screening methods and antitumor evaluation
  Mitomycin C purchased from AbMole

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  FASEB J. 2025 Aug 30;39(17):e71003.

  AIM2‐Silenced Dendritic Cells Possess Immunosuppressive Phenotype and Prolong Allograft Survival by Modulating the Th1/Th17/Treg Balance
  Mitomycin C purchased from AbMole

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  Stem Cell Res Ther. 2024 Sep 11;15(1):295.

  Macrophage extracellular vesicle-packaged miR-23a-3p impairs maintenance and angiogenic capacity of human endothelial progenitor cells in neonatal hyperoxia-induced lung injury
  Mitomycin C purchased from AbMole

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  Bio-protocol. 2024 Aug 07.

  Extraction of Bacterial Membrane Vesicle and Phage Complex by Density Gradient Ultracentrifugation
  Mitomycin C purchased from AbMole

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  Front Immunol. 2023 May 1;14:1182851.

  Inhibition of DAI refrains dendritic cells from maturation and prolongs murine islet and skin allograft survival
  Mitomycin C purchased from AbMole

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  Mol Pharm. 2023 Oct 30.

  Extracellular Vesicles Derived from Human Umbilical Cord MSC Improve Vascular Endothelial Function in In Vitro and In Vivo Models of Preeclampsia through Activating Arginine Metabolism
  Mitomycin C purchased from AbMole

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  Immun Inflamm Dis. 2023 Sep;11(9):e1011.

  Dendritic cells infected with recombinant adenoviral vector encoding mouse fibroblast activation protein-α and human livin α exert an antitumor effect against Lewis lung carcinoma in mice
  Mitomycin C purchased from AbMole

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  Skin Res Technol. 2023 Feb;29(2):e13290.

  The use of human‐derived feeder layers for the cultivation of transplantable human epidermal cell sheet to repair second degree burn wounds
  Mitomycin C purchased from AbMole

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  Comput Math Methods Med. 2022 Jul 13;2022:2517463.

  MicroRNA-424-5p Alleviates Isoflurane Anesthesia-Induced Neurotoxicity in Human Embryonic Stem Cell-Derived Neurons by Targeting FASN
  Mitomycin C purchased from AbMole

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  Patent. CN114085812B 2022 Apr 19.

  Patent. CN114085812B
  Mitomycin C purchased from AbMole

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  Biomed Res Int. 2021 Jun 10;2021:6616547.

  Antioral Squamous Cell Carcinoma Effects of Carvacrol via Inhibiting Inflammation, Proliferation, and Migration Related to Nrf2/Keap1 Pathway
  Mitomycin C purchased from AbMole

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  Patent. CN113559094A 2021 Oct 29.

  Patent. CN113559094A
  Mitomycin C purchased from AbMole

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  Int J Mol Sci. 2020 Apr 30;21(9):3164.

  Leukamenin E Induces K8/18 Phosphorylation and Blocks the Assembly of Keratin Filament Networks Through ERK Activation
  Mitomycin C purchased from AbMole

Customer Product Validations & Biological Datas

	Source	Int J Mol Sci. 2020 Apr. Figure 9. Mitomycin C (Abmole Bioscience, USA)
	Method	Wound healing assays
	Cell Lines	human pancreatic cancer cells (Panc-1)
	Concentrations	10 ug/mL
	Incubation Time	12 h
	Results	Leukamenin E induced an inhibitory e ect on the migration of HUVECs and Panc-1 cells using both transwell and wound healing assay.

Protocol (for reference only)

Cell Experiment
Cell lines	CD34+ EPCs
Preparation method	Briefly, treated CD34+ EPCs were seeded in 12-well plates to create a confluent monolayer. Once they reached 90% confluency, the cell monolayer was firstly treated with mitomycin c (final concentration at 10 µg/mL; AbMole Bioscience #M5791) for 2 h before scratching with a sterile p100 pipette tip, followed by cell washing with PBS twice before changing to complete EGM.
Concentrations	10 µg/mL
Incubation time	2 h

Animal Experiment
Animal models	Female athymic nude foxn1nu mice
Formulation	Saline
Dosages	20 µg/mL (3 mL)
Administration	Intraperitoneal infusion (40 min)

Chemical Information

Molecular Weight	334.37
Formula	C15H18N4O5
CAS Number	50-07-7
Solubility (25°C)	DMSO 50 mg/mL
Storage	-20°C, protect from light, sealed

References

[1] Jieping Deng, et al. J Leukoc Biol. Identification of HLA-A2 restricted CD8+ T cell epitopes in SARS-CoV-2 structural proteins

[2] Michelle Vang Mouritzen, et al. J Vis Exp . Optimized Scratch Assay for In Vitro Testing of Cell Migration with an Automated Optical Camera

[3] Laura Dittmar, et al. Hum Immunol. Immunosuppressive properties of mitomycin C-incubated human myeloid blood cells (MIC) in vitro

[4] Rustagi T, et al. J Clin Gastroenterol. Treatment of Refractory Gastrointestinal Strictures With Mitomycin C: A Systematic Review.

[5] Antonio Di Grazia, et al. Antimicrob Agents Chemother . Temporins A and B stimulate migration of HaCaT keratinocytes and kill intracellular Staphylococcus aureus

[6] Christian Andreas Radu, et al. J Surg Res. Mitomycin-C-treated peripheral blood mononuclear cells (PBMCs) prolong allograft survival in composite tissue allotransplantation

[7] Weiwen Yang, et al. Int J Cancer. Kinetics of repression by modified p53 on the PDGF beta-receptor promoter

[8] Jeanho Yun, et al. Oncogene. Hypersensitivity of Brca1-deficient MEF to the DNA interstrand crosslinking agent mitomycin C is associated with defect in homologous recombination repair and aberrant S-phase arrest