All AbMole products are for research use only, cannot be used for human consumption.
Insulin (human) is a polypeptide hormone that regulates the level of sugar (glucose) in the blood and is produced by beta cells of islets.
*The product is unstable in the solution state, so it is recommended that you use it now and use it as soon as possible
This product solution cannot be autoclaved.
It is also soluble in 125mM NaHCO3. Alkaline storage solutions are not recommended as high pH increases deamidation and protein aggregation.
General recommendation:
1. Do not prepare concentrated insulin solution because of poor solubility and the formation of polymers.
2. Adjust acidic pH to promote solutization and minimize the effects of neutral ph-binding proteases. For added protection, acid protease inhibitors may be considered.
3. The solution was aseptically filtered using a 0.2 micron low protein binding filter. Avoid aeration and/or blistering during solubilization and filtration as this may lead to protein denaturation.
4. Store at 2-8°C for 8 hours.
5. Do not heat more than 30°C and do not freeze.
6. Recommended working concentration of insulin is 1-10 μg/mL.
As an alternative, insulin solutions can be formulated with phosphate buffers (PBS). The above products were dissolved in 1mg /mL PBS solution. Shake gently at room temperature for 1-2 hours. No whirlpool. The solution is ready for cleanup. Prior to evaluation, follow steps 3-6 for final processing.
Sci Adv. 2025 Jul 25;11(30).
Human iPSC-based breast cancer model identifies S100P-dependent cancer stemness induced by BRCA1 mutation
Insulin (human) purchased from AbMole
Food Res Int. 2023 Feb 14.
N-acetylcysteine promotes the proliferation of porcine adipose-derived stem cells during in vitro long-term expansion for cultured meat production
Insulin (human) purchased from AbMole
Biorxiv. 2023 Dec.
GTPase-activating protein ARAP1 regulates circular dorsal ruffles as a nutrient uptake mechanism in the Hep3B hepatocellular carcinoma cell line
Insulin (human) purchased from AbMole
Cell Commun Signal. 2022 Jul 7;20(1):102.
Circular dorsal ruffles disturb the growth factor-induced PI3K-AKT pathway in hepatocellular carcinoma Hep3B cells
Insulin (human) purchased from AbMole
| Cell Experiment | |
|---|---|
| Cell lines | 3T3-L1 and human SGBS cells |
| Preparation method | To model the effects of hyperinsulinemia in vitro and characterize the effects of prolonged insulin exposure in adipocytes, we incubated mouse 3T3-L1 and human SGBS to a supraphysiological dose of insulin (150 nM), which was added to the medium every day from day 6 post differentiation induction until differentiation to mature adipocytes. |
| Concentrations | 150 nM |
| Incubation time | 4 days |
| Animal Experiment | |
|---|---|
| Animal models | Diabetic rats that underwent I/R |
| Formulation | Not mentioned |
| Dosages | 2~12 IU/kg |
| Administration | Tail vein injection |
| Molecular Weight | 5807.57 |
| Formula | C257H383N65O77S6 |
| CAS Number | 11061-68-0 |
| Solubility (25°C) | Water 10 mg/mL (Need ultrasonic and warming and adjust pH to 2-3 with HCl) |
| Storage | -20°C, protect from light, dry, sealed |
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All AbMole products are for research use only, cannot be used for human consumption or veterinary use. We do not provide products or services to individuals. Please comply with the intended use and do not use AbMole products for any other purpose.
Products are for research use only. Not for human use. We do not sell to patients.
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