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(+)-Bicuculline

Cat. No. M2347

All AbMole products are for research use only, cannot be used for human consumption.

(+)-Bicuculline Structure
Synonym:

d-Bicuculline

Size Price Availability Quantity
10mM*1mL in DMSO USD 50  USD50 In stock
20mg USD 36  USD36 In stock
50mg USD 63  USD63 In stock
100mg USD 95  USD95 In stock
250mg USD 180  USD180 In stock
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Quality Control & Documentation
Biological Activity

(+)-Bicuculline (1-100μM) dose-dependently inhibits the Cl- conductance generated by 40 μM GABA. (+)-Bicuculline inhibits GABA(A) α2/β1/γ2 currents activated by γ-Aminobutyric acid, (+)-Bicuculline concentration from 10 nM to 2 μM progressively decreased the amplitude of GABA-activated ion currents. Furthermore, (+)-bicuculine also blocks small-conductance calcium-activated potassium channels.

Product Citations
Protocol (for reference only)
Cell Experiment
Cell lines Primary cortical neurons from rat E18 embryos
Preparation method Bicuculline treatment could potentiate synaptic NMDAR signaling in the primary cortical neurons. Bicuculline led to a rapid decrease in STEP61 expression and a concomitant increase in the tyrosine phosphorylation of STEP substrates including GluN2B, Pyk2, and ERK1/2.
Concentrations 10 μM
Incubation time 15-60 min
Animal Experiment
Animal models Fischer-344 virgin female rats of Ovariectomy
Formulation sesame oil + 10% DMSO
Dosages 3.5 mg/kg daily
Administration via osmotic subcutaneous pumps
Chemical Information
Molecular Weight 367.35
Formula C20H17NO6
CAS Number 485-49-4
Solubility (25°C) DMSO ≥ 40 mg/mL
Storage Powder          -20°C   3 years ;  4°C   2 years
In solvent       -80°C   6 months ;  -20°C   1 month
References

[1] Braden BB, et al. Front Aging Neurosci. The GABAA antagonist bicuculline attenuates progesterone-induced memory impairments in middle-aged ovariectomized rats.

[2] Huang SH, et al. Eur J Pharmacol. Bilobalide, a sesquiterpene trilactone from Ginkgo biloba, is an antagonist at recombinant alpha1beta2gamma2L GABA(A) receptors.

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Keywords: (+)-Bicuculline, d-Bicuculline supplier, GABA Receptor, inhibitors, activators

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